Proline: Mother Nature's cryoprotectant applied to protein crystallography
نویسندگان
چکیده
منابع مشابه
Proline: Mother Nature's cryoprotectant applied to protein crystallography.
L-Proline is one of Mother Nature's cryoprotectants. Plants and yeast accumulate proline under freeze-induced stress and the use of proline in the cryopreservation of biological samples is well established. Here, it is shown that L-proline is also a useful cryoprotectant for protein crystallography. Proline was used to prepare crystals of lysozyme, xylose isomerase, histidine acid phosphatase a...
متن کاملDetritus: Mother Natures Rice Cake
While todays popular opinion appears to simply discount the importance of detritus as just plain mud, scientific evidence indicates that the ancient cultures may have been correct concerning the importance of detritus. Detritus is important on a global scale as well as locally; from its role in the world carbon cycle to supplying part of the nutritional requirements of a marsh periwinkle (St...
متن کاملL-proline: a highly effective cryoprotectant for mouse oocyte vitrification
Recent studies have shown that L-proline is a natural osmoprotectant and an antioxidant to protect cells from injuries such as that caused by freezing and thawing in many species including plant, ram sperm and human endothelial cells. Nevertheless, this nontoxic cryoprotectant has not yet been applied to mammalian oocyte vitrification. In this study we evaluated the efficiency and safety of the...
متن کاملDatabases in protein crystallography.
Applications of structural databases in the protein crystallographic structure determination process are reviewed, using mostly examples from work carried out by the authors. Four application areas are discussed: model building, model refinement, model validation and model analysis.
متن کاملHigh-pressure protein crystallography and NMR to explore protein conformations.
High-pressure methods for solving protein structures by X-ray crystallography and NMR are maturing. These techniques are beginning to impact our understanding of thermodynamic and structural features that define not only the protein's native conformation, but also the higher free energy conformations. The ability of high-pressure methods to visualize these mostly unexplored conformations provid...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Crystallographica Section D Biological Crystallography
سال: 2012
ISSN: 0907-4449
DOI: 10.1107/s0907444912019580